Ephrin-B2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84830
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMP
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 21539827). Use in Rat reported in scientific literature (PMID:28755882).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ephrin-B2 Antibody
Western Blot: Ephrin-B2 Antibody [NBP1-84830]
Western Blot: Ephrin-B2 Antibody [NBP1-84830] - Colonic levels of EphB4 and ephrin-B2 proteins. Representative western blots showing the expression of EphB4, ephrin-B2 and beta-actin proteins in lysates, obtained from the colon of vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT) or EphB4 20 ug/kg (EphB4). Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fphar.2019.00691/full), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830]
Immunocytochemistry/Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830]
Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human placenta shows moderate membranous positivity in endothelial cells.Applications for Ephrin-B2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 21539827). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ephrin-B2
Alternate Names
EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1
Gene Symbol
EFNB2
Additional Ephrin-B2 Products
Product Documents for Ephrin-B2 Antibody
Product Specific Notices for Ephrin-B2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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