Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2G4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all epithelial Sodium Channel alpha Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)

Localization

Apical Cell Membrane

Specificity

Detects 85kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for epithelial Sodium Channel alpha Antibody (2G4)

Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Western Blot: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salf diet. Load: 20 ug. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~85kDa.
Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red.
Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382]

Immunohistochemistry: epithelial Sodium Channel alpha Antibody (2G4) [NBP2-59382] - Immunohistochemistry analysis using Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody, Clone 2G4 (NBP2-59382). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-epithelial Sodium Channel alpha Monoclonal Antibody (NBP2-59382) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Applications for epithelial Sodium Channel alpha Antibody (2G4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: epithelial Sodium Channel alpha

Epithelial Sodium Channel alpha (SCNN1A) is a subunit of the epithelial sodium channel (ENaC). ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. The alpha subunit supports sodium conductance when expressed alone; the beta and gamma subunits do not support sodium conductance by themselves, but greatly augment the channel activity when expressed in conjunction with the alpha subunit. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).

Long Name

Amiloride-sensitive sodium channel subunit alpha

Alternate Names

Alpha-ENaC, Alpha-NaCH, ENaCA, SCNEA, SCNN1, SCNN1A

Gene Symbol

SCNN1A

UniProt

P37088 (Human)

Additional epithelial Sodium Channel alpha Products

Product Documents for epithelial Sodium Channel alpha Antibody (2G4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel alpha Antibody (2G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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