epithelial Sodium Channel alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16353
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human epithelial Sodium Channel alpha. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (85%), Rat (85%), Bovine (85%).
Localization
Apical cell membrane; Multi-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for epithelial Sodium Channel alpha Antibody
![Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353]](https://resources.bio-techne.com/images/products/epithelial-Sodium-Channel-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-16353-img0002.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353]")
Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353]
Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] -
Immunohistochemistry-Paraffin: epithelial Sodium Channel alpha Antibody [NBP2-16353] -Mouse kidney.ENaC Alpha stained by ENaC Alpha antibody [N3C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: epithelial Sodium Channel alpha Antibody [NBP2-16353] -
Western Blot: epithelial Sodium Channel alpha Antibody [NBP2-16353] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [N3C2], Internal (NBP2-16353) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for epithelial Sodium Channel alpha Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: epithelial Sodium Channel alpha
Long Name
Amiloride-sensitive sodium channel subunit alpha
Alternate Names
Alpha-ENaC, Alpha-NaCH, ENaCA, SCNEA, SCNN1, SCNN1A
Gene Symbol
SCNN1A
UniProt
Additional epithelial Sodium Channel alpha Products
Product Documents for epithelial Sodium Channel alpha Antibody
Product Specific Notices for epithelial Sodium Channel alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.