EPLIN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38673

Novus Biologicals, part of Bio-Techne
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NBP2-38673
NBP2-38673-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EPLIN Antibodies »

Product Summary for EPLIN Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EPLIN Antibody

Western Blot: EPLIN Antibody [NBP2-38673]

Western Blot: EPLIN Antibody [NBP2-38673]

Western Blot: EPLIN Antibody [NBP2-38673] - Analysis using Anti-LIMA1 antibody NBP2-38673 (A) shows similar pattern to independent antibody NBP1-87947 (B).
Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP2-38673]

Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP2-38673]

Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP2-38673] - Staining of human cell line U-2 OS shows localization to plasma membrane, actin filaments and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673]

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673]

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human rectum using Anti-LIMA1 antibody NBP2-38673.
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Applications for EPLIN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPLIN

EPLIN is a cytoskeleton associated protein that inhibits actin filament depolymerization and crosslinks filaments in bundles.

Alternate Names

1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3

Gene Symbol

LIMA1

UniProt

Q9UHB6 (Human)

Additional EPLIN Products

Product Documents for EPLIN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EPLIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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