Eps8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-819
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SGVESFDEGSSH corresponding to C-Terminus according to NP_004438.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Eps8 Antibody
Western Blot: Eps8 Antibody [NB300-819] -
Western Blot: Eps8 Antibody [NB300-819] - (1ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] -
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] -
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - (8ug/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Eps8 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
6-8 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1-3 ug/ml
Application Notes
WB: Approx. 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 91.9kDa according to NP_004438.3). An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Eps8
Long Name
Epidermal Growth Factor Receptor Pathway Substrate 8
Alternate Names
epidermal growth factor receptor kinase substrate 8, epidermal growth factor receptor pathway substrate 8
Gene Symbol
EPS8
UniProt
Additional Eps8 Products
Product Documents for Eps8 Antibody
Product Specific Notices for Eps8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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