Epsin-2 Antibody (OTI1G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01775

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G3

Concentration

0.73 mg/ml

View all Epsin-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Epsin-2 Antibody (OTI1G3)

Western Blot: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Western Blot: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Western Blot: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Epsin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Epsin-2.
Immunocytochemistry/ Immunofluorescence: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Immunocytochemistry/ Immunofluorescence: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Immunocytochemistry/Immunofluorescence: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Epsin-2.
Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775]

Immunohistochemistry-Paraffin: Epsin-2 Antibody (OTI1G3) [NBP2-01775] - Staining of paraffin-embedded Human tonsil using anti-Epsin-2 mouse monoclonal antibody.
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Applications for Epsin-2 Antibody (OTI1G3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Epsin-2

This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Alternate Names

Eps15 binding protein, EPS-15-interacting protein 2, epsin 2, epsin-2, KIAA1065EHB21

Entrez Gene IDs

22905 (Human)

Gene Symbol

EPN2

Additional Epsin-2 Products

Product Documents for Epsin-2 Antibody (OTI1G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Epsin-2 Antibody (OTI1G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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