ER alpha/NR3A1 Antibody (33)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-560

Novus Biologicals, part of Bio-Techne
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NB300-560
NB300-560-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian, Bovine, Gerbil, Primate

Cited:

Human, Rat, Gerbil

Applications

Validated:

Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 33

Concentration

1 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.

Reactivity Notes

Gerbil reactivity reported in scientific literature (PMID: 31753310).

Localization

Nuclear

Specificity

Detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha. This does not cross react with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

**For Western blot QC, 50 ug bovine liver was analyzed. Blocking was in 5% milk in PBST for 1 hour. NB300-560 diluted to 1ug/ml was incubated with blocking buffer for 1 hour RT. Three x 8 minute washes were performed, then secondary antibody added, repeat washes, then ECL reagents used. Film exposure determined positive signal.

Scientific Data Images for ER alpha/NR3A1 Antibody (33)

Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560]

Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560]

Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560]

Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560]

Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).

Applications for ER alpha/NR3A1 Antibody (33)

Application
Recommended Usage

Immunofluorescence

10 ug/ml

Immunohistochemistry

1:20 - 1:100

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

4 ug/ml
Application Notes
Blocking usage was reported in scientific literature (PMID: 2360217).

Reviewed Applications

Read 1 review rated 1 using NB300-560 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Dulbecco's PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human); 13982 (Mouse); 24890 (Rat); 407238 (Bovine)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (33)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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