ER alpha/NR3A1 Antibody (ESR1/420)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45005
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # ESR1/420
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human ER alpha/NR3A1 protein (aa290-560) (Uniprot: P03372)
Epitope
aa300-550
Localization
Nucleus
Specificity
This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this monoclonal antibody is mapped between aa300-550. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Marker
Marker of Estrogen Dependence
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-48011)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for ER alpha/NR3A1 Antibody (ESR1/420)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ER alpha/NR3A1
Additional ER alpha/NR3A1 Products
Product Documents for ER alpha/NR3A1 Antibody (ESR1/420)
Product Specific Notices for ER alpha/NR3A1 Antibody (ESR1/420)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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