ER alpha/NR3A1 Antibody (h-151)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-13538
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # h-151
Concentration
1.0 mg/ml
Product Summary for ER alpha/NR3A1 Antibody (h-151)
Immunogen
Synthetic peptide derived from the sequence of human Estrogen Receptor, conjugated to KLH
Reactivity Notes
Human and Rat.
Localization
Nuclear
Specificity
Binding specificity was confirmed in peptide inhibition studies with the intact protein. This does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ER alpha/NR3A1 Antibody (h-151)
![Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538]](https://resources.bio-techne.com/images/products/ER-alpha-NR3A1-Antibody-h-151-Western-Blot-NB120-13538-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538]")
Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538]
Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).Applications for ER alpha/NR3A1 Antibody (h-151)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
15 ug/ml
Western Blot
1:250
Application Notes
Detects a band of ~67 kDa by Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ER alpha/NR3A1
Long Name
Estrogen Receptor alpha
Alternate Names
ESR1, NR3A1
Gene Symbol
ESR1
UniProt
Additional ER alpha/NR3A1 Products
Product Documents for ER alpha/NR3A1 Antibody (h-151)
Product Specific Notices for ER alpha/NR3A1 Antibody (h-151)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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