ER alpha/NR3A1 Antibody (h-151)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-13538

Novus Biologicals, part of Bio-Techne
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NB120-13538
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # h-151

Concentration

1.0 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from the sequence of human Estrogen Receptor, conjugated to KLH

Reactivity Notes

Human and Rat.

Localization

Nuclear

Specificity

Binding specificity was confirmed in peptide inhibition studies with the intact protein. This does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ER alpha/NR3A1 Antibody (h-151)

Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538]

Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538]

Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

Applications for ER alpha/NR3A1 Antibody (h-151)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

15 ug/ml

Western Blot

1:250
Application Notes
Detects a band of ~67 kDa by Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human); 13982 (Mouse); 24890 (Rat)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (h-151)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ER alpha/NR3A1 Antibody (h-151)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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