ER alpha/NR3A1 Antibody (SPM567)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32965

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM567

Concentration

0.2 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Summary for ER alpha/NR3A1 Antibody (SPM567)

Immunogen

Recombinant human ER alpha/NR3A1 protein (aa2-185) (Uniprot: P03372)

Localization

Nuclear

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.

Marker

Marker of Estrogen Dependence

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34801)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER alpha/NR3A1 Antibody (SPM567)

Western Blot: ER alpha/NR3A1 Antibody (SPM567) [NBP2-32965]

Western Blot: ER alpha/NR3A1 Antibody (SPM567) [NBP2-32965]

Western Blot: ER alpha/NR3A1 Antibody (SPM567) [NBP2-32965] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).

Applications for ER alpha/NR3A1 Antibody (SPM567)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (SPM567)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ER alpha/NR3A1 Antibody (SPM567)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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