ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34801
![Western Blot: ER alpha/NR3A1 Antibody (SPM567)Azide and BSA Free [NBP2-34801]](https://resources.bio-techne.com/images/products/ER-alpha-NR3A1-Antibody-SPM567-Azide-and-BSA-Free-Western-Blot-NBP2-34801-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (SPM567)Azide and BSA Free [NBP2-34801]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM567
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
Immunogen
Recombinant human ER alpha/NR3A1 protein (aa2-185) (Uniprot: P03372)
Localization
Nuclear
Specificity
This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.
Marker
Marker of Estrogen Dependence
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32965).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
Western Blot: ER alpha/NR3A1 Antibody (SPM567)Azide and BSA Free [NBP2-34801]
Western Blot: ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (SPM567).
Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] -
Flow Cytometry: Mouse Monoclonal ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free [NBP2-34801] - Analysis using the Alexa Fluor (R) 594 conjugate of NBP2-34801.Clusters of CAF markers were used to identify the fibroblast population in mice tumor model. The ER-alpha expressing patterns in the small group were checked. The Fluor is 594, which was set as BV 605 in BD machine. Image from verified customer review.Applications for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Validated for Flow Cytometry from a verified customer review.
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.
Western Blot: 1-2ug/ml for 2 hours at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ER alpha/NR3A1
Long Name
Estrogen Receptor alpha
Alternate Names
ESR1, NR3A1
Gene Symbol
ESR1
Additional ER alpha/NR3A1 Products
Product Documents for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
Product Specific Notices for ER alpha/NR3A1 Antibody (SPM567) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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