ER alpha/NR3A1 [p Ser106] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81912

Novus Biologicals, part of Bio-Techne
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NB100-81912
NB100-81912-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Summary for ER alpha/NR3A1 [p Ser106] Antibody

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human ER alpha/NR3A1 around the phosphorylation site of serine 106 (S-P- SP-P-L).

Modification

p Ser106

Specificity

Detects endogenous levels of ER alpha/NR3A1 only when phosphorylated at serine 106.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ER alpha/NR3A1 [p Ser106] Antibody

Western Blot: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Western Blot: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Western Blot: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Western blot analysis of extracts from MCF7 cells untreated or treated with EGF
Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Staining of methanol-fixed MCF cells using Estrogen Receptor-alpha (Phospho-Ser106) antibody.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser106] Antibody [NB100-81912] - Analysis of paraffin- embedded human breast carcinoma tissue using Estrogen Receptor-alpha (phospho-Ser106) antibody NB100-81912.
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Applications for ER alpha/NR3A1 [p Ser106] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human); 13982 (Mouse)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 [p Ser106] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 [p Ser106] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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