Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 595) of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-158, NB100-1567, NB100-58824, NB100-97831, NB200-171, NB100-2254, NB100-58826, NB600-233, NB100-314, NB100-77315, NB100-77315

Scientific Data Images for ER alpha/NR3A1 Antibody

Western Blot: ER alpha/NR3A1 Antibody [NB200-347]

Western Blot: ER alpha/NR3A1 Antibody [NB200-347]

Western Blot: ER alpha/NR3A1 Antibody [NB200-347] - Detection of Human Estrogen Receptor Alpha by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody NB200-347 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ER alpha/NR3A1 Antibody [NB200-347]

Immunoprecipitation: ER alpha/NR3A1 Antibody [NB200-347]

Immunoprecipitation: ER alpha/NR3A1 Antibody [NB200-347] - Detection of human ER alpha/NR3A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ER alpha/NR3A1 antibody NB200-347 (lot 3) used for IP at 6 ug per reaction. ER alpha/NR3A1 was also immunoprecipitated by previous lot of this antibody (lot 2). For blotting immunoprecipitated ER alpha/NR3A1, NB200-347 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ER alpha/NR3A1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:5000-1:15000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ER alpha/NR3A1

The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.[supplied by OMIM]

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Entrez Gene IDs

2099 (Human)

Gene Symbol

ESR1

UniProt

P03372

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ER alpha/NR3A1 Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-158, NB100-1567, NB100-58824, NB100-97831, NB200-171, NB100-2254, NB100-58826, NB600-233, NB100-314, NB100-77315, NB100-77315

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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