ERAB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-887
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CIRLDGAIRMQP corresponding to C-Terminus according to NP_004484.1, NP_001032900.1.
Reactivity Notes
Mouse reactivity determined by Western Blot reported in scientific literature (PMID: 17436268).
Specificity
This antibody is expected to recognise both reported isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ERAB Antibody
![Western Blot: ERAB Antibody [NB300-887]](https://resources.bio-techne.com/images/products/ERAB-Antibody-Western-Blot-NB300-887-img0003.jpg "Western Blot: ERAB Antibody [NB300-887]")
Western Blot: ERAB Antibody [NB300-887]
Western Blot: ERAB Antibody [NB300-887] - Analysis of ERAB / HADH2 in Human Brain lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 0.3ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Applications for ERAB Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.3 - 2 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human brain lysates (calculated MW of 26.9 kDa band according to NP_004484). A minor band of unknown identity was also consistently observed at appr 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ERAB
Alternate Names
17-beta-HSD 10, 17-beta-hydroxysteroid dehydrogenase 10, 17b-HSD10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, ABAD, amyloid-beta peptide binding alcohol dehydrogenase, CAMR, DUPXp11.22, EC 1.1.1.178, EC 1.1.1.35, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, ERAB3-hydroxyacyl-CoA dehydrogenase type-2, HADH2AB-binding alcohol dehydrogenase, hydroxyacyl-Coenzyme A dehydrogenase, type II, hydroxyacyl-Coenzyme Adehydrogenase, type II, hydroxysteroid (17-beta) dehydrogenase 10, mental retardation, X-linked, syndromic 10, MHBD, Mitochondrial ribonuclease P protein 2,3-hydroxyacyl-CoA dehydrogenase type II, Mitochondrial RNase P protein 2, MRPP2HCD2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, short chain dehydrogenase/reductase family 5C, member 1, short chain L-3-hydroxyacyl-CoA dehydrogenase type 2, short chain type dehydrogenase/reductase XH98G2, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, XH98G2
Gene Symbol
HSD17B10
UniProt
Additional ERAB Products
Product Documents for ERAB Antibody
Product Specific Notices for ERAB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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