ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52639
Recombinant Monoclonal Antibody.
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric - Azide and BSA Free [NBP2-52639]](https://resources.bio-techne.com/images/products/ErbB2-Her2-Antibody-4D5-8-trastuzumab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52639-img0001.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric - Azide and BSA Free [NBP2-52639]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 4D5-8 (trastuzumab)
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
Specificity
Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric - Azide and BSA Free [NBP2-52639]
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.Applications
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to ErbB2/Her2 has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1, biosimilar antibody, biosimilars
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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