ErbB2/Her2 [p Tyr1139] Antibody (7N1S0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16960

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16960-100ul
NBP3-16960-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7N1S0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ErbB2/Her2 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y1139 of human ErbB2/Her2 (P04626). PEYVN

Modification

p Tyr 1139

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB2/Her2 [p Tyr1139] Antibody (7N1S0)

Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (7N1S0) [NBP3-16960] - Western blot analysis of extracts of A-431 cells, using ErbB2/Her2 (NBP3-16960) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for ErbB2/Her2 [p Tyr1139] Antibody (7N1S0)

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 [p Tyr1139] Antibody (7N1S0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ErbB2/Her2 [p Tyr1139] Antibody (7N1S0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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