ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44084

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0268

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ErbB2/Her2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P04626, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free

ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Staining of human ErbB2/Her2-positive breast cancer shows strong membranous positivity in tumor cells.
ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Staining of human liver shows no positivity in hepatocytes as expected.
ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-44084]

Staining of human ErbB2/Her2-negative breast cancer shows no positivity in tumor cells, as expected.
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Applications for ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ErbB2/Her2 Antibody (CL0268) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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