ErbB2/Her2 Antibody (HRB2/451)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29437

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-29437
NBP2-29437-20ug
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HRB2/451

Concentration

0.2 mg/ml

View all ErbB2/Her2 Antibodies »

Product Specifications

Immunogen

Recombinant human ErbB2/Her2 protein (Uniprot: IDP04626)

Localization

Cell Surface

Specificity

This monoclonal antibody is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33064)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ErbB2/Her2 Antibody (HRB2/451)

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).
Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437]

Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
View 2 more images

Applications for ErbB2/Her2 Antibody (HRB2/451)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
ELISA: For coating, order antibody without BSA)
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ErbB2/Her2

Also known as erbB2 and CD340, HER-2 is closely related in structure to the epidermal growth factor receptor. It is over-expressed in a variety of carcinomas, especially those of breast and ovary.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

UniProt

P04626

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody (HRB2/451)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ErbB2/Her2 Antibody (HRB2/451)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.