ErbB2/Her2 Antibody (trastuzumab) - Humanized
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27964
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # trastuzumab
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
ERBB2 / HER2 / CD340
Reactivity Notes
Predicted species reactivity: Mouse
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
< 0.001EU/ug,determined by LAL method.
Description
Expressed from CHO. It has a predicted MW of 145.5 kDa.
Also known as 'trastuzumab'.
Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
Also known as 'trastuzumab'.
Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ErbB2/Her2 Antibody (trastuzumab) - Humanized
In vivo assay: ErbB2/Her2 Antibody (trastuzumab) - Humanized[NBP3-27964] -
Trastuzumab inhibited the tumor growth of BT474 on NCG mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 88.5% at 10 mpk at D35.
ELISA: ErbB2/Her2 Antibody (trastuzumab) - Humanized[NBP3-27964] -
Immobilized human HER2, His at 2 ug/mL can bind ErbB2/Her2 Antibody (trastuzumab) - Humanized, EC50=0.005735 ug/mL.
Flow Cytometry: ErbB2/Her2 Antibody (trastuzumab) - Humanized[NBP3-27964] -
BT474 cells were stained with ErbB2/Her2 Antibody (trastuzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.3024 ug/mL.Applications for ErbB2/Her2 Antibody (trastuzumab) - Humanized
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
Formulation
Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 Antibody (trastuzumab) - Humanized
Product Specific Notices for ErbB2/Her2 Antibody (trastuzumab) - Humanized
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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