ErbB2/Her2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-81957
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human ErbB2/Her2 around amino aicds 1246~1250 (P-E-Y-L-G).
Specificity
Detects endogenous levels of total ErbB2/Her2 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ErbB2/Her2 Antibody - BSA Free
Western Blot: ErbB2/Her2 Antibody [NB100-81957] -
Western Blot: ErbB2/Her2 Antibody [NB100-81957] - Analysis of extracts from MCF and SKOV3 cells NB100-81957
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-81957] -
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-81957] - Staining of paraffin-embedded human breast carcinoma tissue using NB100-81957(left) or the same antibody preincubated with blocking peptide(right).
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NB100-81957] -
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NB100-81957] - Staining of methanol-fixed MCF7 cells using NB100-81957Applications for ErbB2/Her2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:50-1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 Antibody - BSA Free
Product Specific Notices for ErbB2/Her2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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