ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-81961
![Western Blot: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961]](https://resources.bio-techne.com/images/products/ErbB2-Her2-[p-Tyr877]-Antibody-Western-Blot-NB100-81961-img0001.jpg "Western Blot: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ErbB2/Her2 around the phosphorylation site of tyrosine 877 (T-E-YP-H-A).
Modification
p Tyr877
Specificity
Detects endogenous levels of ErbB2/Her2 only when phosphorylated at tyrosine 877.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
![ErbB2/Her2 [p Tyr877] Antibody - BSA Free](https://resources.bio-techne.com/images/products/nb100-81961_rabbit-polyclonal-erbb2-her2-p-tyr877-antibody-113202594525.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -")
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81961 incubated with blocking peptide (right)![ErbB2/Her2 [p Tyr877] Antibody - BSA Free](https://resources.bio-techne.com/images/products/nb100-81961_rabbit-polyclonal-erbb2-her2-p-tyr877-antibody-113202515145437.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -")
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] -
Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr877] Antibody [NB100-81961] - Staining of methanol-fixed MCF7 cells using NB100-81961Applications for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
Product Specific Notices for ErbB2/Her2 [p Tyr877] Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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