ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13232
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1221/Tyr1222 of human ErbB2/Her2. The exact sequence is proprietary.
Modification
p Tyr 1221, p Tyr 1222
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody
![Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]](https://resources.bio-techne.com/images/products/ErbB2-Her2-[p-Tyr1222--p-Tyr1221]-Antibody-Western-Blot-NBP3-13232-img0001.jpg "Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]")
Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]
Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]](https://resources.bio-techne.com/images/products/ErbB2-Her2-[p-Tyr1222--p-Tyr1221]-Antibody-Immunohistochemistry-Paraffin-NBP3-13232-img0002.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]")
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232]
Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody detects ErbB2/Her2 (phospho Tyr1221/Tyr1222) protein at cell membrane by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right). Sample: Paraffin-embedded human breast carcinoma. ErbB2/Her2 (phospho Tyr1221/Tyr1222) stained by ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Documents for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody
Product Specific Notices for ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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