ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70661

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ErbB3/Her3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ERBB3 (NP_001973) produced in SF9 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free

Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Flow Cytometry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Flow Cytometry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Flow Cytometry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661] - HEK293T cells transfected with either ErbB3/Her3 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostaining by anti-ERBB3 antibody , and then analyzed by flow cy
Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661]

Immunohistochemistry: ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free [NBP2-70661] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ErbB3/Her3

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ErbB3/Her3 Antibody (OTI3E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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