Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Summary for ErbB3/Her3 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1292 to 1342 of human V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 using the numbering given in entry NP_001973.2 (GeneID 2065).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB3/Her3 Antibody

Western Blot: ErbB3/Her3 Antibody [NBP2-32253]

Western Blot: ErbB3/Her3 Antibody [NBP2-32253]

Western Blot: ErbB3/Her3 Antibody [NBP2-32253] - Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, Jurkat, and MCF7 cells. Antibodies: Affinity purified rabbit anti-ErbB3 antibody NBP2-32253 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ErbB3/Her3 Antibody [NBP2-32253]

Immunoprecipitation: ErbB3/Her3 Antibody [NBP2-32253]

Immunoprecipitation: ErbB3/Her3 Antibody [NBP2-32253] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from MCF7 cells. Antibodies: Affinity purified rabbit anti-ErbB3 antibody NBP2-32253 used for IP at 6 ug per reaction. For blotting immunoprecipitated ErbB3, NBP2-32253 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ErbB3/Her3 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-32253 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ErbB3/Her3

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Gene Symbol

ERBB3

UniProt

NP_001973.2 (Human)

Additional ErbB3/Her3 Products

Product Documents for ErbB3/Her3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ErbB3/Her3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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