ErbB4/Her4 Antibody (H4.77.16 (Ab77))
Novus Biologicals, part of Bio-Techne | Catalog # NB120-3104
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # H4.77.16 (Ab77)
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Type I membrane protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for ErbB4/Her4 Antibody (H4.77.16 (Ab77))
Western Blot: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]
Western Blot: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of 25 ug of mouse brain cell lysates.Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in T47D cells (green) compared to an isotype control (blue).Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16 (Ab77)) [NB120-3104]
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in MCF-7 cells (green) compared to an isotype control (blue).Applications for ErbB4/Her4 Antibody (H4.77.16 (Ab77))
Application
Recommended Usage
Flow Cytometry
0.5 ug
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunoprecipitation
2 ug/mg
Western Blot
1:50 - 1:500
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28267150).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ErbB4/Her4
Long Name
Receptor Tyrosine Protein Kinase ErbB4
Alternate Names
HER4
Gene Symbol
ERBB4
UniProt
Additional ErbB4/Her4 Products
Product Documents for ErbB4/Her4 Antibody (H4.77.16 (Ab77))
Product Specific Notices for ErbB4/Her4 Antibody (H4.77.16 (Ab77))
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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