ERCC1 Antibody (8F1) [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-704JF646

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB500-704AF350

Alexa Fluor 532

NB500-704AF532

Alexa Fluor 594

NB500-704AF594

Alexa Fluor 750

NB500-704AF750

Biotin

NB500-704B

DyLight 350

NB500-704UV

DyLight 405

NB500-704V

DyLight 488

NB500-704G

DyLight 550

NB500-704R

DyLight 594

NB500-704DL594

DyLight 650

NB500-704C

DyLight 680

NB500-704FR

DyLight 755

NB500-704IR

FITC

NB500-704F

HRP

NB500-704H

Janelia Fluor 549

NB500-704JF549

mFluor Violet 450 SE

NB500-704MFV450

mFluor Violet 500 SE

NB500-704MFV500

mFluor Violet 610 SE

NB500-704MFV610

Forumulation

Catalog #

BSA Free

NB500-704

Azide and BSA Free

NBP2-80725

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat, Chinese Hamster

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 646

Antibody Source

Monoclonal Mouse IgG_2B Clone # 8F1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERCC1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human ERCC1. [UniProt# P07992]

Reactivity Notes

Human, rat. Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for ERCC1 Antibody (8F1) [Janelia Fluor® 646]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (8F1) [Janelia Fluor® 646]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for ERCC1 Antibody (8F1) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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