ERCC1 Antibody (8F1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80725
![Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]](https://resources.bio-techne.com/images/products/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80725-img0001.jpg "Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Chinese Hamster
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 8F1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for ERCC1 Antibody (8F1) - Azide and BSA Free
Immunogen
Full length recombinant human ERCC1. [UniProt# P07992]
Reactivity Notes
Chinese Hamster reactivity reported in scientific literature (PMID: 17962301) Not yet tested in other species.
Localization
Nuclear.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ERCC1 Antibody (8F1) - Azide and BSA Free
Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]
Immunohistochemistry: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]](https://resources.bio-techne.com/images/products/ERCC1-Antibody-8F1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80725-img0002.jpg "Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]")
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725]
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) - Azide and BSA Free [NBP2-80725] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody. Image from the standard format of this antibody.Applications for ERCC1 Antibody (8F1) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500. Use reported in scientific literature (PMID 21961533)
Western Blot
1:100-1:2000. Use reported in scientific literature
Application Notes
IHC: In an ABC method, we suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERCC1
Long Name
Excision Repair Cross-complementing 1
Alternate Names
COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20
Gene Symbol
ERCC1
Additional ERCC1 Products
Product Documents for ERCC1 Antibody (8F1) - Azide and BSA Free
Product Specific Notices for ERCC1 Antibody (8F1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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