Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ERCC8 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ERCC8(NP_001007235) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERCC8 Antibody (OTI2G1) - Azide and BSA Free

Western Blot: ERCC8 Antibody (OTI2G1)Azide and BSA Free [NBP2-70412]

Western Blot: ERCC8 Antibody (OTI2G1)Azide and BSA Free [NBP2-70412]

Western Blot: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8.
Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412]

Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412]

Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412]

Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412]

Immunohistochemistry: ERCC8 Antibody (OTI2G1) - Azide and BSA Free [NBP2-70412] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for ERCC8 Antibody (OTI2G1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERCC8

ERCC8 encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

Alternate Names

CKN1DNA excision repair protein ERCC-8, Cockayne syndrome 1 (classical), Cockayne syndrome WD repeat protein CSA, CSACockayne syndrome WD-repeat protein CSA, excision repair cross-complementing rodent repair deficiency, complementationgroup 8

Gene Symbol

ERCC8

Additional ERCC8 Products

Product Documents for ERCC8 Antibody (OTI2G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERCC8 Antibody (OTI2G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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