ERCC8 Antibody (PSH0-86)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32315
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH0-86
Concentration
1 mg/ml
Product Summary for ERCC8 Antibody (PSH0-86)
Immunogen
Recombinant protein within human ERCC8 aa 1-396 / 396. (Uniprot: Q13216)
Localization
Nucleus, Nucleus matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERCC8 Antibody (PSH0-86)
Western Blot: ERCC8 Antibody (PSH0-86) [NBP3-32315]
Western Blot: ERCC8 Antibody (PSH0-86) [NBP3-32315] - Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (NBP3-32315) at 1/1,000 dilution.Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: HepG2 cell lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 44 kDa
Observed band size: 55 kDa
Exposure time: 27 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32315) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for ERCC8 Antibody (PSH0-86)
Application
Recommended Usage
Western Blot
1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERCC8
Alternate Names
CKN1DNA excision repair protein ERCC-8, Cockayne syndrome 1 (classical), Cockayne syndrome WD repeat protein CSA, CSACockayne syndrome WD-repeat protein CSA, excision repair cross-complementing rodent repair deficiency, complementationgroup 8
Gene Symbol
ERCC8
Additional ERCC8 Products
Product Documents for ERCC8 Antibody (PSH0-86)
Product Specific Notices for ERCC8 Antibody (PSH0-86)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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