eRF1 Antibody (2H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00002107-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eRF1 Antibodies »

Product Specifications

Immunogen

ETF1 (NP_004721.1, 338 a.a. ~ 437 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDY

Specificity

ETF1 - eukaryotic translation termination factor 1 (2H4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for eRF1 Antibody (2H4)

Western Blot: eRF1 Antibody (2H4) [H00002107-M02]

Western Blot: eRF1 Antibody (2H4) [H00002107-M02]

Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - Analysis of ETF1 expression in transfected 293T cell line by ETF1 monoclonal antibody (M02), clone 2H4.Lane 1: ETF1 transfected lysate (Predicted MW: 49 KDa).Lane 2: Non-transfected lysate.
Western Blot: eRF1 Antibody (2H4) [H00002107-M02]

Western Blot: eRF1 Antibody (2H4) [H00002107-M02]

Western Blot: eRF1 Antibody (2H4) [H00002107-M02] - ETF1 monoclonal antibody (M02), clone 2H4. Analysis of ETF1 expression in HepG2.
Sandwich ELISA: eRF1 Antibody (2H4) [H00002107-M02] - Detection limit for recombinant GST tagged ETF1 is approximately 1ng/ml as a capture antibody.

Applications for eRF1 Antibody (2H4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eRF1

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Alternate Names

D5S1995, ERF1eRF1, eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eukaryotic translation termination factor 1, polypeptide chain release factor 1, Protein Cl1, RF1ERF, sup45 (yeast omnipotent suppressor 45) homolog-like 1, SUP45L1, TB3-1MGC111066

Entrez Gene IDs

2107 (Human)

Gene Symbol

ETF1

UniProt

NP_004721 (Human)

Additional eRF1 Products

Product Documents for eRF1 Antibody (2H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for eRF1 Antibody (2H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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