eRF1 Antibody (4F9H12) - BSA Free

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using eRF1 mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).

ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] -

Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 & CC-90009 on COL17A1 PTC readthrough. (A, B) HaCaT keratinocytes derived from an unaffected individual were exposed to various concentrations of CC-885 (A) or CC-90009 (B) with or without 100 μg/ml gentamicin sulfate for 48 h & cell viability was measured using the MTT assay in triplicate samples (±SD). Concentrations of CC-885 & CC-90009 are in nanomolar. (C, D) JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-885 (C) or CC-90009 (D) for 72 h & eRF3a, eRF3b & eRF1 levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. (E, F) JEB01 keratinocytes were exposed to the indicated concentrations of CC-885 (E) or CC-90009 (F) in the presence or absence of 100 or 300 μg/ml gentamicin sulfate for 72 h & Collagen XVII levels were measured by SDS-PAGE followed by traditional western blotting. beta-actin was used as loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33764477), licensed under a CC-BY license. Not internally tested by Novus Biologicals.