ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67555
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC58-01
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human aa 166-215 / 379. (SwissProt: P27361 Human; SwissProt: P28482 Human; SwissProt: P63085 Mouse; SwissProt: Q63844 Mouse; SwissProt: P21708 Rat; SwissProt: P63086 Rat)
Modification
p Thr202, p Tyr204, p Thr185, p Tyr187
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01)
Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Analysis of ERK1 (pT202/pY204)+ERK2 (pT185/pY187) on Jurkat cell lysates.Lane 1: Jurkat cells, whole cell lysate, 10ug/lane, Lane 2/3: Jurkat cells treated with 200ng/ml PMA for 30 minutes, whole cell lysates, 10ug/lane, Lane 4: Jurkat cells treated with 200ng/ml PMA for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-ERK1 (pT202/pY204)+ERK2 (pT185/pY187) antibody () at 1:500 dilution. Anti-ERK1+ERK2 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution.Predicted band size:41/43 kDaObserved band size:41/43 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes 32 seconds.
Immunohistochemistry-Paraffin: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Human thyroid carcinoma tissue with Rabbit anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01) [NBP2-67555] - Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra
Applications for ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01)
Application
Recommended Usage
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:500-1:2000
Application Notes
IP-Use at an assay dependent concentration.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERK1/2
Alternate Names
EC 2.7.11, ERK-1, ERK1p44-MAPK, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MGC20180, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, PRKM3EC 2.7.11.24
Gene Symbol
MAPK3
Additional ERK1/2 Products
Product Documents for ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01)
Product Specific Notices for ERK1/2 [p Thr202, p Tyr204, p Thr185, p Tyr187] Antibody (SC58-01)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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