ERK1 Antibody (0B0C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15776

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15776-100ul
NBP3-15776-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0B0C1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERK1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERK1 (P27361). MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK1 Antibody (0B0C1)

Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunohistochemistry of paraffin-embedded human lung cancer using ERK1 Rabbit mAb (NBP3-15776) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunohistochemistry-Paraffin: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunohistochemistry of paraffin-embedded human colon carcinoma using ERK1 Rabbit mAb (NBP3-15776) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776]

Immunoprecipitation: ERK1 Antibody (0B0C1) [NBP3-15776] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug ERK1 antibody (NBP3-15776). Western blot was performed from the immunoprecipitate using ERK1 antibody (NBP3-15776) at a dilition of 1:1000.
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Applications for ERK1 Antibody (0B0C1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK1

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

Additional ERK1 Products

Product Documents for ERK1 Antibody (0B0C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK1 Antibody (0B0C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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