Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1A2

Format

BSA Free

Concentration

1 mg/ml

View all ERK1 Antibodies »

Product Specifications

Immunogen

Recombinant human ERK1 protein (1-137aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ERK1 Antibody (1A2) - BSA Free

Western Blot: ERK1 Antibody (1A2)BSA Free [NBP1-74032]

Western Blot: ERK1 Antibody (1A2)BSA Free [NBP1-74032]

Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: HeLa cell lysate
Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (1A2) - BSA Free [NBP1-74032]

Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (1A2) - BSA Free [NBP1-74032]

Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: ERK1 Antibody (1A2)BSA Free [NBP1-74032]

Western Blot: ERK1 Antibody (1A2)BSA Free [NBP1-74032]

Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MAPK1 recombinant protein Lane 2.: MAPK3 recombinant protein

Applications for ERK1 Antibody (1A2) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK1

Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

UniProt

NP_002737.2 (Human)

Additional ERK1 Products

Product Documents for ERK1 Antibody (1A2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK1 Antibody (1A2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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