Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D7

Concentration

1 mg/ml

View all ERK1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAPK3(NP_002737) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK1 Antibody (OTI4D7)

Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814]

Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814]

Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814]

Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814]

Immunohistochemistry: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of Adenocarcinoma of Human ovary tissue.
Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814]

Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814]

Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.
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Applications for ERK1 Antibody (OTI4D7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK1

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

Additional ERK1 Products

Product Documents for ERK1 Antibody (OTI4D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK1 Antibody (OTI4D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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