ERK1/ERK2 [p Tyr204] Antibody (SR1718)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21522

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21522-100ul
NBP3-21522-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1718

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ERK1/ERK2 Antibodies »

Product Summary for ERK1/ERK2 [p Tyr204] Antibody (SR1718)

Immunogen

A synthesized peptide derived from human phospho-ERK1 [Tyr204]/ERK2 [Tyr187] (Uniprot #: P27361, P28482)

Modification

p Tyr 204

Specificity

Detects endogenous levels of total phospho-ERK1 [Tyr204]/ERK2 [Tyr187]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK1/ERK2 [p Tyr204] Antibody (SR1718)

Western Blot: ERK1/ERK2 [p Tyr 204] Antibody (SR1718) [NBP3-21522] -

Western Blot: ERK1/ERK2 [p Tyr 204] Antibody (SR1718) [NBP3-21522] - Western blot analysis using NBP3-21522 in A431 cell lysate treated with EGF.

Applications for ERK1/ERK2 [p Tyr204] Antibody (SR1718)

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK1/ERK2

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3

Gene Symbol

MAPK3

Additional ERK1/ERK2 Products

Product Documents for ERK1/ERK2 [p Tyr204] Antibody (SR1718)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERK1/ERK2 [p Tyr204] Antibody (SR1718)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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