ERK2 Antibody (4C11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60506

Novus Biologicals, part of Bio-Techne
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NB110-60506-0.025ml
NB110-60506
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4C11

Format

BSA Free

Concentration

1 mg/ml

View all ERK2 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ERK2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

ERK2 (4C11C11C4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK2 Antibody (4C11) - BSA Free

Western Blot: ERK2 Antibody (4C11)BSA Free [NB110-60506]

Western Blot: ERK2 Antibody (4C11)BSA Free [NB110-60506]

Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (4C11) [NB110-60506] - Analysis of Eca-109 cells using ERK2 mouse mAb (green).
Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) - BSA Free [NB110-60506]

Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) [NB110-60506] - Analysis of human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.

Applications for ERK2 Antibody (4C11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK2

ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Entrez Gene IDs

5594 (Human)

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (4C11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK2 Antibody (4C11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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