ERK2 Antibody (5-D2-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32317
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 5-D2-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human ERK2 aa aa 200-359. (Uniprot: P28482)
Localization
Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERK2 Antibody (5-D2-R)
Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317]
Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Jurkat cell lysate (20 ug/Lane)
Lane 3: A549 cell lysate (20 ug/Lane)
Lane 4: A431 cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: RAW264.7 cell lysate (20 ug/Lane)
Lane 9: C6 cell lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (20 ug/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Applications for ERK2 Antibody (5-D2-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERK2
Long Name
Extracellular Signal-regulated Kinase 2
Alternate Names
ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2
Gene Symbol
MAPK1
Additional ERK2 Products
Product Documents for ERK2 Antibody (5-D2-R)
Product Specific Notices for ERK2 Antibody (5-D2-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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