ERK2 Antibody (PCRP-MAPK1-1D1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13754

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MAPK1-1D1

Concentration

0.2 mg/ml

View all ERK2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ERK2 protein (Uniprot: P28482)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14157)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ERK2 Antibody (PCRP-MAPK1-1D1)

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]

Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).

Applications for ERK2 Antibody (PCRP-MAPK1-1D1)

Application
Recommended Usage

Flow Cytometry

0.5-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

UniProt

P28482

Additional ERK2 Products

Product Documents for ERK2 Antibody (PCRP-MAPK1-1D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK2 Antibody (PCRP-MAPK1-1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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