ERK2 Antibody (PCRP-MAPK1-1D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13754
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-MAPK1-1D1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human ERK2 protein (Uniprot: P28482)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14157)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ERK2 Antibody (PCRP-MAPK1-1D1)
Immunocytochemistry/ Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]
Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754]
Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).Applications for ERK2 Antibody (PCRP-MAPK1-1D1)
Application
Recommended Usage
Flow Cytometry
0.5-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ERK2
Long Name
Extracellular Signal-regulated Kinase 2
Alternate Names
ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2
Gene Symbol
MAPK1
UniProt
Additional ERK2 Products
Product Documents for ERK2 Antibody (PCRP-MAPK1-1D1)
Product Specific Notices for ERK2 Antibody (PCRP-MAPK1-1D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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