Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ERK2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 310 and 360 of human extracellular signal-regulated kinase-2 (mitogen-activated protein kinase 1) using the numbering given in entry NP_002736.3 (GeneID 5594).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-312, NB100-314

Scientific Data Images for ERK2 Antibody

Western Blot: ERK2 Antibody [NBP1-19102]

Western Blot: ERK2 Antibody [NBP1-19102]

Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.
Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102]

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.

Applications for ERK2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ERK2

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Entrez Gene IDs

5594 (Human)

Gene Symbol

MAPK1

UniProt

NP_002736.3 (Human)

Additional ERK2 Products

Product Documents for ERK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERK2 Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-312, NB100-314

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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