Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ERK3 Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

Western Blot: ERK3/MAPK6 Antibody (OTI5E1)Azide and BSA Free [NBP2-71242]

Western Blot: ERK3/MAPK6 Antibody (OTI5E1)Azide and BSA Free [NBP2-71242]

Western Blot: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242]

Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242]

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.
Immunohistochemistry: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242]

Immunohistochemistry: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242]

Immunohistochemistry: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.

Applications for ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK3

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Gene Symbol

MAPK6

Additional ERK3 Products

Product Documents for ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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