ERK3/MAPK6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84806

Novus Biologicals, part of Bio-Techne
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NBP1-84806
NBP1-84806-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERK3 Antibodies »

Product Summary for ERK3/MAPK6 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YSFPMDEPISSHPFHIEDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYSDHHENKYCDLECSH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK3/MAPK6 Antibody

Western Blot: ERK3/MAPK6 Antibody [NBP1-84806]

Western Blot: ERK3/MAPK6 Antibody [NBP1-84806]

Western Blot: ERK3/MAPK6 Antibody [NBP1-84806] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806]

Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806]

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP1-84806]

Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP1-84806]

Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP1-84806] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Applications for ERK3/MAPK6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK3

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Gene Symbol

MAPK6

Additional ERK3 Products

Product Documents for ERK3/MAPK6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERK3/MAPK6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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