ERManI Antibody (3C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13167

Novus Biologicals, part of Bio-Techne
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NBP2-13167
NBP2-13167SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C2

Concentration

1.0 mg/ml

View all ERManI Antibodies »

Product Specifications

Immunogen

Full length human recombinant ERManI [Swiss-Prot# Q9UKM7]

Reactivity Notes

Does not react with mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ERManI Antibody (3C2)

Western Blot: ERManI Antibody (3C2) [NBP2-13167]

Western Blot: ERManI Antibody (3C2) [NBP2-13167]

Western Blot: ERManI Antibody (3C2) [NBP2-13167] - Western blot analysis of ERManI expression in 1) NTERA-2 and 2) A-431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: ERManI Antibody (3C2) [NBP2-13167]

Immunocytochemistry/ Immunofluorescence: ERManI Antibody (3C2) [NBP2-13167]

Immunocytochemistry/Immunofluorescence: ERManI Antibody (3C2) [NBP2-13167] - ERManI antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: ERManI Antibody (3C2) [NBP2-13167]

Immunohistochemistry: ERManI Antibody (3C2) [NBP2-13167]

Immunohistochemistry: ERManI Antibody (3C2) [NBP2-13167] - IHC staining of ERManI in human breast cancer using DAB with hematoxylin counterstain.

Applications for ERManI Antibody (3C2)

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:100

Knockout Validated

reported in scientific literature (PMID 26205822)

Western Blot

1:500-1:1000
Application Notes
In ICC/IF, endoplasmic reticulum membrane staining was observed in HeLa cells. In Western Blot, a band is seen ~90 kDa representing ERManI. In IHC-P, staining was observed in the endoplasmic reticulum of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine (pH 7.5) and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERManI

ERManI, also known as endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase or MAN1B1, is a putative ER resident mannosidase that plays a rate-limiting role in generating a signal that targets misfolded N-linked glycoproteins for ER-associated degradation (ERAD). ERManI codes for an enzyme that converts Man9GlcNAc to Man8GlcNAc isomer B during N-glycan biosynthesis. It also facilitates the trimming of Man5-6GlcNAc2 in the endoplasmic reticulum associated degradation pathway. Multiple transcript variants of ERManI can result from alternative splicing, and a related pseudogene can be found on chromosome 11. Research has found that mutations on the ERManI gene can result in autosomal-recessive intellectual disabilities and developmental delays (PMID: 21763484). ERManI is predominately localized to the Golgi and endoplasmic reticulum.

Alternate Names

alpha 1,2-mannosidase, EC 3.2.1.113, endoplasmic reticulum alpha-mannosidase 1, endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase, endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1, ER alpha 1,2-mannosidase, ER alpha-1,2-mannosidase, ER ManI, ER mannosidase 1, ERMan1, Man9GlcNAc2-specific processing alpha-mannosidase, Man9GlcNAc2-specific-processing alpha-mannosidase, MANA-ER, Mannosidase alpha class 1B member 1, mannosidase, alpha, class 1B, member 1, MRT15, RP11-350O14.2, MAN1B1

Entrez Gene IDs

11253 (Human)

Gene Symbol

MAN1B1

UniProt

Q9UKM7 (Human)

Additional ERManI Products

Product Documents for ERManI Antibody (3C2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERManI Antibody (3C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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