ERO1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84800

Novus Biologicals, part of Bio-Techne
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NBP1-84800
NBP1-84800-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERO1L alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERO1L Antibody

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800]

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800]

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800] - Staining in human esophagus and skeletal muscle tissues using anti-ERO1A antibody. Corresponding ERO1A RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800]

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800]

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800] - Staining of human cerebral cortex, colon, esophagus and testis using Anti-ERO1A antibody NBP1-84800 (A) shows similar protein distribution across tissues to independent antibody NBP1-84799 (B).
Western Blot: ERO1L Antibody [NBP1-84800]

Western Blot: ERO1L Antibody [NBP1-84800]

Western Blot: ERO1L Antibody [NBP1-84800] - Analysis using Anti-ERO1A antibody NBP1-84800 (A) shows similar pattern to independent antibody NBP1-84799 (B).
View 6 more images

Applications for ERO1L Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERO1L alpha

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

Long Name

Endoplasmic Reticulum Oxidoreductin 1 alpha

Alternate Names

ERO1-alpha, ERO1-L, ERO1-l alpha, ERO1-like, ERO1-like alpha, ERO1A, ERO1L

Gene Symbol

ERO1A

Additional ERO1L alpha Products

Product Documents for ERO1L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERO1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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