ERp72 Antibody (2H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00009601-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERp72 Antibodies »

Product Specifications

Immunogen

PDIA4 (NP_004902, 355 a.a. ~ 464 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKD

Specificity

PDIA4 - protein disulfide isomerase family A, member 4 (2H3)

Marker

Endoplasmic Reticulum Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ERp72 Antibody (2H3)

Western Blot: ERp72 Antibody (2H3) [H00009601-M02]

Western Blot: ERp72 Antibody (2H3) [H00009601-M02]

Western Blot: ERp72 Antibody (2H3) [H00009601-M02] - Analysis of PDIA4 expression in transfected 293T cell line by PDIA4 monoclonal antibody (M02), clone 2H3.Lane 1: PDIA4 transfected lysate (Predicted MW: 72.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ERp72 Antibody (2H3) [H00009601-M02] - Detection limit for recombinant GST tagged PDIA4 is approximately 3ng/ml as a capture antibody.

Applications for ERp72 Antibody (2H3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERp72

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Alternate Names

Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ERP70ER protein 72, ERp72, ERp-72, ERP72EC 5.3.4.1, intestinal-related), protein disulfide isomerase family A, member 4, protein disulfide isomerase related protein (calcium-binding protein, protein disulfide isomerase-associated 4, protein disulfide-isomerase A4

Entrez Gene IDs

9601 (Human)

Gene Symbol

PDIA4

UniProt

NP_004902 (Human)

Additional ERp72 Products

Product Documents for ERp72 Antibody (2H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERp72 Antibody (2H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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