Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # EPO/1368

Concentration

0.2 mg/ml

View all Erythropoietin/EPO Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 28-162) of human Erythropoietin/EPO protein (exact sequence is proprietary) (Uniprot: P01588)

Localization

Cytoplasmic and Cell Surface

Marker

Marker of Placentation Disorders

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54537)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Erythropoietin/EPO Antibody (EPO/1368)

Western Blot: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Western Blot: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Western Blot: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392] - Western Blot of human Heart and HepG2 cell lysate using Erythropoietin/EPO Antibody (EPO/1368).
Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Erythropoietin/EPO Antibody (EPO/1368).
Protein Array: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Protein Array: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392]

Protein Array: Erythropoietin/EPO Antibody (EPO/1368) [NBP2-53392] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Erythropoietin/EPO Antibody (EPO/1368).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Erythropoietin/EPO Antibody (EPO/1368)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Erythropoietin/EPO

Erythropoietin (Epo) stimulates red blood cell production by docking with its cognate receptor on the erythroid progenitor cell and triggering an array of signaling pathways that inhibit apoptosis and promote cell proliferation and differentiation. In its pharmaceutical forms, epoetin and darbepoetin, Epo is widely used to treat various anemias, including those associated with cancer.

Alternate Names

ECYT5, EPO, MVCD2

Gene Symbol

EPO

UniProt

P01588

Additional Erythropoietin/EPO Products

Product Documents for Erythropoietin/EPO Antibody (EPO/1368)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Erythropoietin/EPO Antibody (EPO/1368)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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