ESD Antibody (J0-8-R)

Western Blot: ESD Antibody (J0-8-R) [NBP3-32319]

Western Blot: ESD Antibody (J0-8-R) [NBP3-32319] - Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (NBP3-32319) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: SW480 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 1 minute 13 seconds;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.