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Eukaryotic translation initiation factor 5B Antibody (3F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009669-M02

Novus Biologicals, part of Bio-Techne
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H00009669-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EIF5B (NP_056988, 1121 a.a. ~ 1218 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFE

Specificity

EIF5B - eukaryotic translation initiation factor 5B (3F9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Eukaryotic translation initiation factor 5B Antibody (3F9)

Sandwich ELISA: Eukaryotic translation initiation factor 5B Antibody (3F9) [H00009669-M02] - Detection limit for recombinant GST tagged EIF5B is 0.1 ng/ml as a capture antibody.

Applications for Eukaryotic translation initiation factor 5B Antibody (3F9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is useful for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Eukaryotic translation initiation factor 5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq]

Alternate Names

DKFZp434I036, eukaryotic translation initiation factor 5B, FLJ10524, IF2Translation initiation factor IF-2, KIAA0741eIF-5B, translation initiation factor IF2

Entrez Gene IDs

9669 (Human)

Gene Symbol

EIF5B

Additional Eukaryotic translation initiation factor 5B Products

Product Documents for Eukaryotic translation initiation factor 5B Antibody (3F9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Eukaryotic translation initiation factor 5B Antibody (3F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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