Eukaryotic translation initiation factor 5B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55202

Novus Biologicals, part of Bio-Techne
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NBP2-55202
NBP2-55202-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Eukaryotic translation initiation factor 5B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKE

Reactivity Notes

Mouse 81%, Rat 81%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Eukaryotic translation initiation factor 5B Antibody

Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP2-55202]

Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP2-55202]

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP2-55202] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Applications for Eukaryotic translation initiation factor 5B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Eukaryotic translation initiation factor 5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq]

Alternate Names

DKFZp434I036, eukaryotic translation initiation factor 5B, FLJ10524, IF2Translation initiation factor IF-2, KIAA0741eIF-5B, translation initiation factor IF2

Gene Symbol

EIF5B

Additional Eukaryotic translation initiation factor 5B Products

Product Documents for Eukaryotic translation initiation factor 5B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Eukaryotic translation initiation factor 5B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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