Eukaryotic translation initiation factor 5B Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03412

Novus Biologicals, part of Bio-Techne
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NBP3-03412-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Eukaryotic translation initiation factor 5B Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human Eukaryotic translation initiation factor 5B (NP_056988.3). MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Eukaryotic translation initiation factor 5B AntibodyAzide and BSA Free [NBP3-03412]

Western Blot: Eukaryotic translation initiation factor 5B AntibodyAzide and BSA Free [NBP3-03412]

Western Blot: Eukaryotic translation initiation factor 5B Antibody [NBP3-03412] - Analysis of extracts of various cell lines, using Eukaryotic translation initiation factor 5B antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody - Azide and BSA Free [NBP3-03412]

Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody - Azide and BSA Free [NBP3-03412]

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP3-03412] - Analysis of U-2 OS cells using Eukaryotic translation initiation factor 5B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody - Azide and BSA Free [NBP3-03412]

Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody - Azide and BSA Free [NBP3-03412]

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 5B Antibody [NBP3-03412] - Analysis of C6 cells using Eukaryotic translation initiation factor 5B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Eukaryotic translation initiation factor 5B

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq]

Alternate Names

DKFZp434I036, eukaryotic translation initiation factor 5B, FLJ10524, IF2Translation initiation factor IF-2, KIAA0741eIF-5B, translation initiation factor IF2

Gene Symbol

EIF5B

Additional Eukaryotic translation initiation factor 5B Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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